Overall process of analysis in Metaflow¶

graph LR
    subgraph Account
        A(Register) --> B(Login)
    end
    Account --> File
    subgraph File
        C(Create a Project) --> D(Create an Experiment)
        D --> E(Upload Files)
    end
    File --> Analysis
    subgraph Analysis
        F(Create a Workflow)
        F --> G(Clean)
        G --> H(Cluster)
    end
    Analysis --> I(Meta-analysis)

To start analyzing your FCS files with Metaflow, go to the Metaflow application website: https://apps.metaflow.bio/

1. Account: Log in your account.
   • Register for an account if you don't have one already.
   • Reset your password if you forgot it.
2. File management: Upload and manage the files you want to analyze.
   • Create a new "Project" or select an existing Project.
   • Create a new "Experiment" or select an existing Experiment inside your chosen Project.
   • Upload files or select existing files inside your chosen Experiment.
3. Analysis: Identify cell populations of interest in your files.
   • Create a new "Workflow" or select an existing Workflow containing your selected files.
   • "Clean" or isolate particles of interest.
   • "Cluster" or identify cell population of interest.
4. Meta-analysis: Set-up "Meta-analysis" to compare cell populations across files.